DynaPro Dynamic Light Scattering
The DynaPro is a combination Dynamic Light Scattering (DLS) and Static Light Scattering (SLS) instrument. The DLS detector operates at 90 degrees to measure the size distribution of the hydrodynamic radius whereas the independent SLS detector, which also operates at 90 degrees, can measure the molecular weight of small molecules. DynaPro NanoStar can be used for a broad range of applications requiring both accuracy and high sensitivity. For example, the DynaPro NanoStar is ideally suited for studies of nanoparticles, proteins, vesicles, viruses, and colloids.
The DynaPro NanoStar is straightforward to operate. Knowledge of the underlying physics of molecular sizing is not essential. […] The sample is filtered to eliminate dust particles, which might interfere with the signal from the molecules being measured. Solutions are injected into cuvetttes and placed into the sample cell. The total sample injection volume (including filtration) is approximately 20 ul using the 10ul cuvette. After the cuvette is placed in the sample cell, the sample is illuminated by the laser. The scattered light is correlated in the DynaPro NanoStar, which then sends the results to the PC for analysis by the DYNAMICS software package. From the DynaPro NanoStar User’s Guide (M3300 Rev. B), Wyatt Technology Corporation
Phillies, G. D. "Quasielastic Light Scattering", Analytical Chemistry 1990, 62(20), 1049A-1057A.
Wyatt, Philip J. “Light scattering and the absolute characterization of macromolecules”, Analytica Chimica Acta 1993, 272, 1-40.
Beretta, Sabrina; Lunelli, Lorenzo; Chirico, Giuseppe; Baldini, Giancarlo "Dynamic Light Scattering from Small Particles: Expected Accuracy in Hemoglobin Data Reduction", Applied Optics 1996, 35(19), 3763-3770.
Bernstein, Bradley E.; Michels, Paul A. M.; Kim, Hidong; Petra, Philip H.; Hol, Wim G. J. "The Importance of Dynamic Light Scattering in Obtaining Multiple Crystal Forms of Trypanosoma brucei PGK", Protein Science 1998, 7, 504-507.
Zulauf, Martin; D'Arcy, Allan "Light Scattering of Proteins as a Criterion for Crystallization", Journal of Crystal Growth 1992, 122, 102-106.
Ferre-D'Amare, Adrian; Burley, Stephen K. "Dynamic Light Scattering in Evaluating Crystallizability of Macromolecules", Methods in Enzymology 1997, 276, 157-166.
Also see DynaPro Application Notes under Literature on the Wyatt Technology website at http://wyatt.com
The Dynamics 7.1.4 User's Guide is available under Programs, Wyatt Technology, Dynamics 7.1.4.3, Dynamics User's Guide.
VIDEO SHOWING DLS OPERATION
